DGKA recombinant monoclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Hela (A), A549 (B) whole cell lysates with DGKA recombinant monoclonal antibody (Cat # RAB02585).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of human tonsil formalin fixed paraffin embedded tissue section using DGKA recombinant monoclonal antibody (Cat # RAB02585). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.127). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human DGKA.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein of human DGKA.
Theoretical MW (kDa)
80
Reactivity
Human
Specificity
Recognizes endogenous levels of DGK alpha protein.
Form
Liquid
Purification
Immunogen affinity chromatography
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:50-1:100)
Immunoprecipitation (1:10-1:50)
Western Blot (1:500-1:1000)Storage Buffer
In 50mM Tris-Glycine, pH 7.4 (0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Hela (A), A549 (B) whole cell lysates with DGKA recombinant monoclonal antibody (Cat # RAB02585).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of human tonsil formalin fixed paraffin embedded tissue section using DGKA recombinant monoclonal antibody (Cat # RAB02585). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.127). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.Immunoprecipitation
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Gene Info — DGKA
Entrez GeneID
1606Protein Accession#
P23743Gene Name
DGKA
Gene Alias
DAGK, DAGK1, DGK-alpha, MGC12821, MGC42356
Gene Description
diacylglycerol kinase, alpha 80kDa
Omim ID
125855Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the eukaryotic diacylglycerol kinase family. It acts as a modulator that competes with protein kinase C for the second messenger diacylglycerol in intracellular signaling pathways. It also plays an important role in the resynthesis of phosphatidylinositols and phosphorylating diacylglycerol to phosphatidic acid. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified. [provided by RefSeq
Other Designations
diacylglycerol kinase alpha|diacylglycerol kinase, alpha (80kD)
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Interactome
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Pathway
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