FLNB (Phospho S2152/2107) recombinant monoclonal antibody
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human FLNB.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic phosphopeptide corresponding to residues surrounding Ser2152 of human FLNB.
Theoretical MW (kDa)
280
Reactivity
Human, Rat
Specificity
Recognizes endogenous levels of Filamin A/B (pS2152/2107) protein.
Form
Liquid
Purification
Immunogen affinity chromatography
Isotype
IgG
Recommend Usage
Immunocytochemistry (1:50-1:100)
Immunofluorescence (1:50-1:100)
Immunohistochemistry (1:50-1:100)
Western Blot (1:500-1:1000)Storage Buffer
In 50mM Tris-Glycine, pH 7.4 (0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Hela (A), A549 (B), U2OS (C), C6 (D) whole cell lysates with FLNB (Phospho S2152/2107) recombinant monoclonal antibody (Cat # RAB02571).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of human cholangiocarcinoma formalin fixed paraffin embedded tissue section using FLNB (Phospho S2152/2107) recombinant monoclonal antibody (Cat # RAB02571). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.47). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.Immunocytochemistry
Immunofluorescence
Immunofluorescent analysis of A549 cells with FLNB (Phospho S2152/2107) recombinant monoclonal antibody (Cat # RAB02571). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. -
Gene Info — FLNB
Entrez GeneID
2317Protein Accession#
O75369Gene Name
FLNB
Gene Alias
ABP-278, AOI, DKFZp686A1668, DKFZp686O033, FH1, FLN1L, LRS1, SCT, TABP, TAP
Gene Description
filamin B, beta (actin binding protein 278)
Gene Ontology
HyperlinkGene Summary
O
Other Designations
beta filamin|filamin 1 (actin-binding protein-280)-like|filamin B, beta (actin-binding protein-278)
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Interactome
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Pathway
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