SYP recombinant monoclonal antibody
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human SYP.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide of human SYP.
Theoretical MW (kDa)
38
Reactivity
Human, Rat
Specificity
Recognizes endogenous levels of Synaptophysin protein.
Form
Liquid
Purification
Immunogen affinity chromatography
Isotype
IgG
Recommend Usage
Immunocytochemistry (1:50-1:100)
Immunofluorescence (1:50-1:100)
Immunohistochemistry (1:50-1:100)
Western Blot (1:500-1:1000)Storage Buffer
In 50mM Tris-Glycine, pH 7.4 (0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of rat brain (A) whole cell lysates with SYP recombinant monoclonal antibody (Cat # RAB02543).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of human brain formalin fixed paraffin embedded tissue section using SYP recombinant monoclonal antibody (Cat # RAB02543). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.Immunocytochemistry
Immunofluorescence
Immunofluorescent analysis of NIH3T3 cells with SYP recombinant monoclonal antibody (Cat # RAB02543). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. -
Gene Info — SYP
Entrez GeneID
6855Protein Accession#
P08247Gene Name
SYP
Gene Alias
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Gene Description
synaptophysin
Omim ID
313475Gene Ontology
HyperlinkGene Summary
Synaptophysin (p38) is an integral membrane protein of small synaptic vesicles in brain and endocrine cells.[supplied by OMIM
Other Designations
major synaptic vesicle protein P38
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Interactome
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Disease
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