MSN recombinant monoclonal antibody
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More Files
- More Functions
Western Blot
Western blot analysis of mouse heart (A), CHOK1 (B), C6 (C), Jurkat (D) whole cell lysates with Moesin recombinant monoclonal antibody (Cat # RAB02521).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of human lung cancer formalin fixed paraffin embedded tissue section using Moesin recombinant monoclonal antibody (Cat # RAB02521). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.85). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human MSN.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide of human Moesin.
Theoretical MW (kDa)
68
Reactivity
Hamster, Human, Mouse, Rat
Specificity
Recognizes endogenous levels of Moesin protein.
Form
Liquid
Purification
Immunogen affinity chromatography
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:50-1:100)
Immunoprecipitation(1:10-1:50)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50mM Tris-Glycine, pH 7.4 (0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of mouse heart (A), CHOK1 (B), C6 (C), Jurkat (D) whole cell lysates with Moesin recombinant monoclonal antibody (Cat # RAB02521).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of human lung cancer formalin fixed paraffin embedded tissue section using Moesin recombinant monoclonal antibody (Cat # RAB02521). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.85). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.Immunoprecipitation
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Gene Info — MSN
Entrez GeneID
4478Protein Accession#
P26038Gene Name
MSN
Gene Alias
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Gene Description
moesin
Omim ID
309845Gene Ontology
HyperlinkGene Summary
Moesin (for membrane-organizing extension spike protein) is a member of the ERM family which includes ezrin and radixin. ERM proteins appear to function as cross-linkers between plasma membranes and actin-based cytoskeletons. Moesin is localized to filopodia and other membranous protrusions that are important for cell-cell recognition and signaling and for cell movement. [provided by RefSeq
Other Designations
OTTHUMP00000023438
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Interactome
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Pathway
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