Products

Western Blot (Cell lysate)

Western blot analysis of C6 (A) whole cell lysates with GRP78 recombinant monoclonal antibody (Cat # RAB02506).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Immunohistochemical analysis of human breast carcinoma formalin fixed paraffin embedded tissue section using GRP78 recombinant monoclonal antibody (Cat # RAB02506). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.12). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Entrez GeneID

3309

Protein Accession#

P11021

Gene Name

HSPA5

Gene Alias

BIP, FLJ26106, GRP78, MIF2

Gene Description

heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)

Omim ID

138120

Gene Ontology

Hyperlink

Gene Summary

When Chinese hamster K12 cells are starved of glucose, the synthesis of several proteins, called glucose-regulated proteins (GRPs), is markedly increased. Hendershot et al. (1994) [PubMed 8020977] pointed out that one of these, GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER). Because so many ER proteins interact transiently with GRP78, it may play a key role in monitoring protein transport through the cell.[supplied by OMIM

Other Designations

Heat-shock 70kD protein-5 (glucose-regulated protein, 78kD)|OTTHUMP00000022124|heat shock 70kD protein 5 (glucose-regulated protein, 78kD)|heat shock 70kDa protein 5