Products

Western Blot (Cell lysate)

Western blot analysis of Jurkat (A) whole cell lysates with Cyclin E2 recombinant monoclonal antibody (Cat # RAB02487).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Immunohistochemical analysis of human lung cancer formalin fixed paraffin embedded tissue section using Cyclin E2 recombinant monoclonal antibody (Cat # RAB02487). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.80). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunocytochemistry

Immunofluorescence

Immunofluorescent analysis of MCF7 cells with Cyclin E2 recombinant monoclonal antibody (Cat # RAB02487). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.

Entrez GeneID

9134

Protein Accession#

O96020

Gene Name

CCNE2

Gene Alias

CYCE2

Gene Description

cyclin E2

Omim ID

603775

Gene Ontology

Hyperlink

Gene Summary

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2. This cyclin has been shown to specifically interact with CIP/KIP family of CDK inhibitors, and plays a role in cell cycle G1/S transition. The expression of this gene peaks at the G1-S phase and exhibits a pattern of tissue specificity distinct from that of cyclin E1. A significantly increased expression level of this gene was observed in tumor-derived cells. [provided by RefSeq

Other Designations

G1/S-specific cyclin E2