CCNE2 recombinant monoclonal antibody, clone R02-1C0
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More Files
- More Functions
Western Blot
Western Blot analysis of Jurkat lysates with CCNE2 recombinant monoclonal antibody, clone R02-1C0 (Cat # RAB02350).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human lung cancer with CCNE2 recombinant monoclonal antibody, clone R02-1C0 (Cat # RAB02350). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human CCNE2.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human CCNE2.
Theoretical MW (kDa)
Calculated MW: 47 kD
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence(1:50-1:200)
Immunohistochemistry (1:50-1:100)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at -20 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of Jurkat lysates with CCNE2 recombinant monoclonal antibody, clone R02-1C0 (Cat # RAB02350).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human lung cancer with CCNE2 recombinant monoclonal antibody, clone R02-1C0 (Cat # RAB02350). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.Immunohistochemistry (Frozen sections)
Immunofluorescence
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Gene Info — CCNE2
Entrez GeneID
9134Protein Accession#
O96020Gene Name
CCNE2
Gene Alias
CYCE2
Gene Description
cyclin E2
Omim ID
603775Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2. This cyclin has been shown to specifically interact with CIP/KIP family of CDK inhibitors, and plays a role in cell cycle G1/S transition. The expression of this gene peaks at the G1-S phase and exhibits a pattern of tissue specificity distinct from that of cyclin E1. A significantly increased expression level of this gene was observed in tumor-derived cells. [provided by RefSeq
Other Designations
G1/S-specific cyclin E2
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Interactome
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Pathway
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Disease
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