BAG6 recombinant monoclonal antibody
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More Files
- More Functions
Western Blot
Western blot analysis of K562 (A), rat brain (B), C6 (C), NIH3T3 (D) whole cell lysates with BAG6 recombinant monoclonal antibody (Cat # RAB02474).
Immunofluorescence
Immunofluorescent analysis of MCF7 cells with BAG6 recombinant monoclonal antibody (Cat # RAB02474). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human BAG6.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide of human BAT3.
Theoretical MW (kDa)
150
Reactivity
Human, Mouse, Rat
Specificity
Recognizes endogenous levels of BAG6 protein.
Form
Liquid
Purification
Immunogen affinity chromatography
Isotype
IgG
Recommend Usage
Immunocytochemistry (1:50-1:100)
Immunofluorescence (1:50-1:100)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50mM Tris-Glycine, pH 7.4 (0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of K562 (A), rat brain (B), C6 (C), NIH3T3 (D) whole cell lysates with BAG6 recombinant monoclonal antibody (Cat # RAB02474).Immunocytochemistry
Immunofluorescence
Immunofluorescent analysis of MCF7 cells with BAG6 recombinant monoclonal antibody (Cat # RAB02474). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. -
Gene Info — BAT3
Entrez GeneID
7917Protein Accession#
P46379Gene Name
BAT3
Gene Alias
BAG-6, BAG6, D6S52E, G3
Gene Description
HLA-B associated transcript 3
Omim ID
142590Gene Ontology
HyperlinkGene Summary
This gene was first characterized as part of a cluster of genes located within the human major histocompatibility complex class III region. This gene encodes a nuclear protein that is cleaved by caspase 3 and is implicated in the control of apoptosis. In addition, the protein forms a complex with E1A binding protein p300 and is required for the acetylation of p53 in response to DNA damage. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
HLA-B associated transcript-3|OTTHUMP00000029239|large proline-rich protein BAT3|scythe
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Interactome
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Disease
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