LDHA recombinant monoclonal antibody
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More Files
- More Functions
Western Blot
Western blot analysis of Jurkat (A), rat brain (B), C6 (C), Hela (D) whole cell lysates with LDHA recombinant monoclonal antibody (Cat # RAB02445).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human LDHA.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide of human Lactate Dehydrogenase.
Theoretical MW (kDa)
37
Reactivity
Human, Rat
Specificity
Recognizes endogenous levels of LDHA protein.
Form
Liquid
Purification
Immunogen affinity chromatography
Isotype
IgG
Recommend Usage
Immunocytochemistry (1:50-1:100)
Immunofluorescence (1:50-1:100)
Immunohistochemistry (1:50-1:100)
Immunoprecipitation(1:10-1:50)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50mM Tris-Glycine, pH 7.4 (0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Jurkat (A), rat brain (B), C6 (C), Hela (D) whole cell lysates with LDHA recombinant monoclonal antibody (Cat # RAB02445).Immunohistochemistry
Immunocytochemistry
Immunofluorescence
Immunoprecipitation
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Gene Info — LDHA
Entrez GeneID
3939Protein Accession#
P00338Gene Name
LDHA
Gene Alias
LDH-M, LDH1, PIG19
Gene Description
lactate dehydrogenase A
Omim ID
150000Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene. [provided by RefSeq
Other Designations
L-lactate dehydrogenase A|proliferation-inducing gene 19
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Interactome
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Pathway
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Disease
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