IRF1 recombinant monoclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane1: PC12 whole cell lysate, Lane2:Jurkat whole cell lysate with IRF1 recombinant monoclonal antibody (Cat # RAB02393) at 1:1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using IRF1 recombinant monoclonal antibody (Cat # RAB02393). Counter stained with hematoxylin.
Immunofluorescence
Immunofluorescence analysis of U-2 OS cells using IRF1 recombinant monoclonal antibody (Cat # RAB02393) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against recombinant IRF1.
Antibody Species
Rabbit
Theoretical MW (kDa)
37, 48
Reactivity
Human, Mouse, Rat
Specificity
This antibody detects endogenous levels of IRF1 and does not cross-react with related proteins.
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
Immunocytochemistry (1:50-1:100)
Immunofluorescence (1:50-1:100)
Immunohistochemistry (1:50-1:100)
Western Blot (1:500-1:2000)Storage Buffer
In PBS, pH 7.2 (0.02% sodium azide and 50% glycerol)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane1: PC12 whole cell lysate, Lane2:Jurkat whole cell lysate with IRF1 recombinant monoclonal antibody (Cat # RAB02393) at 1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using IRF1 recombinant monoclonal antibody (Cat # RAB02393). Counter stained with hematoxylin.Immunocytochemistry
Immunofluorescence
Immunofluorescence analysis of U-2 OS cells using IRF1 recombinant monoclonal antibody (Cat # RAB02393) at dilution of 1:100. Blue: DAPI for nuclear staining. -
Gene Info — IRF1
Entrez GeneID
3659Protein Accession#
P10914Gene Name
IRF1
Gene Alias
IRF-1, MAR
Gene Description
interferon regulatory factor 1
Gene Ontology
HyperlinkGene Summary
IRF1 encodes interferon regulatory factor 1, a member of the interferon regulatory transcription factor (IRF) family. IRF1 serves as an activator of interferons alpha and beta transcription, and in mouse it has been shown to be required for double-stranded RNA induction of these genes. IRF1 also functions as a transcription activator of genes induced by interferons alpha, beta, and gamma. Further, IRF1 has been shown to play roles in regulating apoptosis and tumor-suppressoion. [provided by RefSeq
Other Designations
interferon regulatory factor-1
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Interactome
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Disease
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