IRF1 recombinant monoclonal antibody, clone R08-1B4
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More Files
- More Functions
Western Blot
Western Blot analysis of Lane 1: K562, Lane 2: C6 and Lane 3: 3T3 lysates with IRF1 recombinant monoclonal antibody, clone R08-1B4 (Cat # RAB02161).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin/PFA-fixed paraffin-embedded sections) of human lung cancer with IRF1 recombinant monoclonal antibody, clone R08-1B4 (Cat # RAB02161). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human IRF1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein corresponding to human IRF1.
Theoretical MW (kDa)
Calculated MW: 37 kD
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence(1:50-1:200)
Immunohistochemistry (1:50-1:100)
Immunoprecipitation(1:20)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at -20 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of Lane 1: K562, Lane 2: C6 and Lane 3: 3T3 lysates with IRF1 recombinant monoclonal antibody, clone R08-1B4 (Cat # RAB02161).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin/PFA-fixed paraffin-embedded sections) of human lung cancer with IRF1 recombinant monoclonal antibody, clone R08-1B4 (Cat # RAB02161). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.Immunofluorescence
Immunoprecipitation
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Gene Info — IRF1
Entrez GeneID
3659Protein Accession#
P10914Gene Name
IRF1
Gene Alias
IRF-1, MAR
Gene Description
interferon regulatory factor 1
Gene Ontology
HyperlinkGene Summary
IRF1 encodes interferon regulatory factor 1, a member of the interferon regulatory transcription factor (IRF) family. IRF1 serves as an activator of interferons alpha and beta transcription, and in mouse it has been shown to be required for double-stranded RNA induction of these genes. IRF1 also functions as a transcription activator of genes induced by interferons alpha, beta, and gamma. Further, IRF1 has been shown to play roles in regulating apoptosis and tumor-suppressoion. [provided by RefSeq
Other Designations
interferon regulatory factor-1
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Interactome
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Disease
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