CCNA2 recombinant monoclonal antibody, clone R02-9B6
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More Files
- More Functions
Western Blot
Western Blot analysis of Lane 1: K562, Lane 2: C6, Lane 3: 3T3 and Lane 4: Hela lysates with CCNA2 recombinant monoclonal antibody, clone R02-9B6 (Cat # RAB02353).
Immunocytochemistry
Immunocytochemical staining of Hela with CCNA2 recombinant monoclonal antibody, clone R02-9B6 (Cat # RAB02353). (A) CCNA2 (green) and (B) DAPI (blue).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human CCNA2.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human CCNA2.
Theoretical MW (kDa)
Calculated MW: 49 kD
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunocytochemistry
Immunofluorescence(1:50-1:200)
Immunohistochemistry (1:50-1:100)
Immunoprecipitation(1:20)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at -20 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of Lane 1: K562, Lane 2: C6, Lane 3: 3T3 and Lane 4: Hela lysates with CCNA2 recombinant monoclonal antibody, clone R02-9B6 (Cat # RAB02353).Immunohistochemistry
Immunocytochemistry
Immunocytochemical staining of Hela with CCNA2 recombinant monoclonal antibody, clone R02-9B6 (Cat # RAB02353). (A) CCNA2 (green) and (B) DAPI (blue).Immunofluorescence
Immunoprecipitation
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Gene Info — CCNA2
Entrez GeneID
890Protein Accession#
P20248Gene Name
CCNA2
Gene Alias
CCN1, CCNA
Gene Description
cyclin A2
Omim ID
123835Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. This cyclin binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions. [provided by RefSeq
Other Designations
cyclin A
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Interactome
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Pathway
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Disease
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