G6PD recombinant monoclonal antibody, clone R02-6B6
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More Files
- More Functions
Western Blot
Western Blot analysis of Lane 1: Hela, Lane 2: A549 and Lane 3: U2OS lysates with G6PD recombinant monoclonal antibody, clone R02-6B6 (Cat # RAB02253).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human G6PD.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein corresponding to human G6PD.
Theoretical MW (kDa)
Calculated MW: 59 kD
Reactivity
Human, Rat
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:50-1:100)
Immunoprecipitation(1:20)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at -20 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of Lane 1: Hela, Lane 2: A549 and Lane 3: U2OS lysates with G6PD recombinant monoclonal antibody, clone R02-6B6 (Cat # RAB02253).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoprecipitation
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Gene Info — G6PD
Entrez GeneID
2539Protein Accession#
P11413Gene Name
G6PD
Gene Alias
G6PD1
Gene Description
glucose-6-phosphate dehydrogenase
Omim ID
305900Gene Ontology
HyperlinkGene Summary
This gene encodes glucose-6-phosphate dehydrogenase. This protein is a cytosolic enzyme encoded by a housekeeping X-linked gene whose main function is to produce NADPH, a key electron donor in the defense against oxidizing agents and in reductive biosynthetic reactions. G6PD is remarkable for its genetic diversity. Many variants of G6PD, mostly produced from missense mutations, have been described with wide ranging levels of enzyme activity and associated clinical symptoms. G6PD deficiency may cause neonatal jaundice, acute hemolysis, or severe chronic non-spherocytic hemolytic anemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000026034|glucose-6-phosphate 1-dehydrogenase|glucose-6-phosphate dehydrogenase, G6PD
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Interactome
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Pathway
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Disease
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