G6PD polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Human cell line RT-4 Lane 2: Human cell line U-251MG sp Lane 3: Human cell line A-431 Lane 4: Human liver tissue Lane 5: Human tonsil tissue with G6PD polyclonal antibody (Cat # PAB29349) at 1:100-1:250 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human testis with G6PD polyclonal antibody (Cat # PAB29349) shows strong cytoplasmic and nuclear positivity in cells of seminiferous duct at 1:2500-1:5000 dilution.
Immunofluorescence
Immunofluorescent staining of human cell line U-2 OS with G6PD polyclonal antibody (Cat # PAB29349) at 1-4 ug/mL concentration shows positivity in cytoplasm.
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant human G6PD.
Immunogen
Recombinant protein corresponding to human G6PD.
Sequence
FQGDAFHQSDTHIFIIMGASGDLAKKKIYPTIWWLFRDGLLPENTFIVGYARSRLTVADIRKQSEPFFKATPEEKLKLEDFFARNSYVAGQYDDAASYQRLNSHMNALH
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:2500-1:5000)
Immunofluorescence (1-4 ug/mL)
Western Blot (1:100-1:250)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Human cell line RT-4 Lane 2: Human cell line U-251MG sp Lane 3: Human cell line A-431 Lane 4: Human liver tissue Lane 5: Human tonsil tissue with G6PD polyclonal antibody (Cat # PAB29349) at 1:100-1:250 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human testis with G6PD polyclonal antibody (Cat # PAB29349) shows strong cytoplasmic and nuclear positivity in cells of seminiferous duct at 1:2500-1:5000 dilution.Immunofluorescence
Immunofluorescent staining of human cell line U-2 OS with G6PD polyclonal antibody (Cat # PAB29349) at 1-4 ug/mL concentration shows positivity in cytoplasm. -
Gene Info — G6PD
Entrez GeneID
2539Gene Name
G6PD
Gene Alias
G6PD1
Gene Description
glucose-6-phosphate dehydrogenase
Omim ID
305900Gene Ontology
HyperlinkGene Summary
This gene encodes glucose-6-phosphate dehydrogenase. This protein is a cytosolic enzyme encoded by a housekeeping X-linked gene whose main function is to produce NADPH, a key electron donor in the defense against oxidizing agents and in reductive biosynthetic reactions. G6PD is remarkable for its genetic diversity. Many variants of G6PD, mostly produced from missense mutations, have been described with wide ranging levels of enzyme activity and associated clinical symptoms. G6PD deficiency may cause neonatal jaundice, acute hemolysis, or severe chronic non-spherocytic hemolytic anemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000026034|glucose-6-phosphate 1-dehydrogenase|glucose-6-phosphate dehydrogenase, G6PD
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Interactome
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Pathway
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Disease
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