IGF2BP3 recombinant monoclonal antibody, clone R04-7F2
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human IGF2BP3.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein corresponding to human IGF2BP3.
Theoretical MW (kDa)
Calculated MW: 64 kD
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunocytochemistry
Immunofluorescence(1:50-1:200)
Immunoprecipitation(1:20)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at -20 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of Lane 1: K562, Lane 2: rat brain, Lane 3: C6, Lane 4: 3T3 and Lane 5: Hela lysates with IGF2BP3 recombinant monoclonal antibody, clone R04-7F2 (Cat # RAB02174).Immunocytochemistry
Immunocytochemical staining of Hela with IGF2BP3 recombinant monoclonal antibody, clone R04-7F2 (Cat # RAB02174). (A) IGF2BP3 (green) and (B) DAPI (blue).Immunofluorescence
Immunoprecipitation
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Gene Info — IGF2BP3
Entrez GeneID
10643Protein Accession#
O00425Gene Name
IGF2BP3
Gene Alias
DKFZp686F1078, IMP-3, IMP3, KOC1, VICKZ3
Gene Description
insulin-like growth factor 2 mRNA binding protein 3
Omim ID
608259Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is primarily found in the nucleolus, where it can bind to the 5' UTR of the insulin-like growth factor II leader 3 mRNA and may repress translation of insulin-like growth factor II during late development. The encoded protein contains several KH domains, which are important in RNA binding and are known to be involved in RNA synthesis and metabolism. A pseudogene exists on chromosome 7, and there are putative pseudogenes on other chromosomes. [provided by RefSeq
Other Designations
IGF II mRNA binding protein 3|IGF-II mRNA-binding protein 3|KH domain containing protein overexpressed in cancer
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Interactome
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Disease
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