MACROH2A1 recombinant monoclonal antibody, clone R04-8E5
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More Files
- More Functions
Western Blot
Western Blot analysis of Lane 1: rat Brain, Lane 2: C6 and Lane 3: 3T3 lysates with MACROH2A1 recombinant monoclonal antibody, clone R04-8E5 (Cat # RAB02122).
Immunocytochemistry
Immunocytochemical staining of Hela with MACROH2A1 recombinant monoclonal antibody, clone R04-8E5 (Cat # RAB02122). (A) MACROH2A1 (green) and (B) DAPI (blue).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human MACROH2A1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human MACROH2A1.
Theoretical MW (kDa)
Calculated MW: 40 kD
Reactivity
Mouse, Rat
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunocytochemistry
Immunofluorescence(1:50-1:200)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at -20 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of Lane 1: rat Brain, Lane 2: C6 and Lane 3: 3T3 lysates with MACROH2A1 recombinant monoclonal antibody, clone R04-8E5 (Cat # RAB02122).Immunocytochemistry
Immunocytochemical staining of Hela with MACROH2A1 recombinant monoclonal antibody, clone R04-8E5 (Cat # RAB02122). (A) MACROH2A1 (green) and (B) DAPI (blue).Immunofluorescence
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Gene Info — H2AFY
Entrez GeneID
9555Protein Accession#
O75367Gene Name
H2AFY
Gene Alias
H2A.y, H2A/y, H2AF12M, H2AFJ, MACROH2A1.1, mH2A1, macroH2A1.2
Gene Description
H2A histone family, member Y
Omim ID
610054Gene Ontology
HyperlinkGene Summary
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a member of the histone H2A family. It replaces conventional H2A histones in a subset of nucleosomes where it represses transcription and participates in stable X chromosome inactivation. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq
Other Designations
histone macroH2A1.1|histone macroH2A1.2
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Interactome
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Pathway
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Disease
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