NRF1 recombinant monoclonal antibody, clone R02-9A7
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More Files
- More Functions
Western Blot
Western blot analysis of Lane 1: C6, Lane 2: 3T3 and Lane 3: Hela lysates with NRF1 recombinant monoclonal antibody, clone R02-9A7 (Cat # RAB02049).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin/PFA-fixed paraffin-embedded sections) of human colon cancer with NRF1 recombinant monoclonal antibody, clone R02-9A7 (Cat # RAB02049).
High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human NRF1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human NRF1.
Theoretical MW (kDa)
Calculated MW: 54 kD
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1:100)
Immunoprecipitation (1:20)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at -20 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: C6, Lane 2: 3T3 and Lane 3: Hela lysates with NRF1 recombinant monoclonal antibody, clone R02-9A7 (Cat # RAB02049).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin/PFA-fixed paraffin-embedded sections) of human colon cancer with NRF1 recombinant monoclonal antibody, clone R02-9A7 (Cat # RAB02049).
High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.Immunohistochemistry (Frozen sections)
Immunofluorescence
Immunoprecipitation
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Gene Info — NRF1
Entrez GeneID
4899Protein Accession#
Q16656Gene Name
NRF1
Gene Alias
ALPHA-PAL
Gene Description
nuclear respiratory factor 1
Omim ID
600879Gene Ontology
HyperlinkGene Summary
This gene encodes a protein that homodimerizes and functions as a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. The protein has also been associated with the regulation of neurite outgrowth. Alternate transcriptional splice variants, which encode the same protein, have been characterized. Additional variants encoding different protein isoforms have been described but they have not been fully characterized. Confusion has occurred in bibliographic databases due to the shared symbol of NRF1 for this gene and for "nuclear factor (erythroid-derived 2)-like 1" which has an official symbol of NFE2L1. [provided by RefSeq
Other Designations
OTTHUMP00000184912|alpha palindromic-binding protein
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Interactome
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Disease
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