NBN recombinant monoclonal antibody, clone R04-7C7
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More Files
- More Functions
Western Blot
Western blot analysis of Hela lysates with NBN recombinant monoclonal antibody, clone R04-7C7 (Cat # RAB02027).
Immunocytochemistry
Immunocytochemical staining of MCF-7 with NBN recombinant monoclonal antibody, clone R04-7C7 (Cat # RAB02027). (A) NBN (green) and (B) DAPI (blue).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human NBN.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human NBN.
Theoretical MW (kDa)
Calculated MW: 85 kD
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunocytochemistry
Immunofluorescence (1:50-1:200)
Immunoprecipitation (1:20)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at -20 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Hela lysates with NBN recombinant monoclonal antibody, clone R04-7C7 (Cat # RAB02027).Immunocytochemistry
Immunocytochemical staining of MCF-7 with NBN recombinant monoclonal antibody, clone R04-7C7 (Cat # RAB02027). (A) NBN (green) and (B) DAPI (blue).Immunofluorescence
Immunoprecipitation
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Gene Info — NBN
Entrez GeneID
4683Protein Accession#
O60934Gene Name
NBN
Gene Alias
AT-V1, AT-V2, ATV, FLJ10155, MGC87362, NBS, NBS1, P95
Gene Description
nibrin
Gene Ontology
HyperlinkGene Summary
Mutations in this gene are associated with Nijmegen breakage syndrome, an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The encoded protein is a member of the MRE11/RAD50 double-strand break repair complex which consists of 5 proteins. This gene product is thought to be involved in DNA double-strand break repair and DNA damage-induced checkpoint activation. [provided by RefSeq
Other Designations
Nijmegen breakage syndrome 1 (nibrin)|cell cycle regulatory protein p95|p95 protein of the MRE11/RAD50 complex
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Interactome
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Pathway
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Disease
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