PIN1 recombinant monoclonal antibody, clone R01-7G8
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More Files
- More Functions
Western Blot
Western blot analysis of Lane 1: Jurkat, Lane 2: rat brain, Lane 3: C6, Lane 4: CHO-K1 and Lane 5: Hela lysates with PIN1 recombinant monoclonal antibody, clone R01-7G8 (Cat # RAB01998).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin/PFA-fixed paraffin-embedded sections) of human breast cancer with PIN1 recombinant monoclonal antibody, clone R01-7G8 (Cat # RAB01998).
High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human PIN1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein corresponding to human PIN1.
Theoretical MW (kDa)
Calculated MW: 18 kD
Reactivity
Hamster, Human, Mouse, Rat
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1:100)
Immunoprecipitation (1:20)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at -20 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: Jurkat, Lane 2: rat brain, Lane 3: C6, Lane 4: CHO-K1 and Lane 5: Hela lysates with PIN1 recombinant monoclonal antibody, clone R01-7G8 (Cat # RAB01998).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin/PFA-fixed paraffin-embedded sections) of human breast cancer with PIN1 recombinant monoclonal antibody, clone R01-7G8 (Cat # RAB01998).
High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.Immunohistochemistry (Frozen sections)
Immunofluorescence
Immunoprecipitation
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Gene Info — PIN1
Entrez GeneID
5300Protein Accession#
Q13526Gene Name
PIN1
Gene Alias
DOD, UBL5
Gene Description
peptidylprolyl cis/trans isomerase, NIMA-interacting 1
Omim ID
601052Gene Ontology
HyperlinkGene Summary
The human PIN1 gene encodes an essential nuclear peptidylprolyl cis-trans isomerase (PPIase; EC 5.2.1.8) involved in regulation of mitosis. PIN1 belongs to a class of PPIases that includes the E. coli parvulin, yeast Ess1, and Drosophila dodo (dod) gene products (Lu et al., 1996 [PubMed 8606777]).[supplied by OMIM
Other Designations
peptidyl-prolyl cis/trans isomerase, NIMA-interacting|prolyl isomerase|protein (peptidyl-prolyl cis/trans isomerase) NIMA-interacting 1|protein (peptidylprolyl cis/trans isomerase) NIMA-interacting 1
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Interactome
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Disease
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