PIN1 monoclonal antibody, clone 3G8
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More Files
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Western Blot (Cell lysate)
Western blot analysis of 293T , HeLa and Jurkat cell lysates (each 50 ug) were resolved by SDS - PAGE , transferred to PVDF membrane and probed with PIN1 monoclonal antibody , clone 3G8 (1 : 500) (Cat # MAB1080). Proteins were visualized using a goat anti - mouse secondary antibody conjugated to HRP and an ECL detection system.
Flow Cytometry
Flow cytometry analysis of Jurkat cells, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).
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Specification
Product Description
Mouse monoclonal antibody raised against full length recombinant PIN1.
Immunogen
Recombinant protein corresponding to amino acids 1-163 of human PIN1.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Isotype
IgG1, kappa
Recommend Usage
ELISA
Flow Cytometry
Western Blot (1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of 293T , HeLa and Jurkat cell lysates (each 50 ug) were resolved by SDS - PAGE , transferred to PVDF membrane and probed with PIN1 monoclonal antibody , clone 3G8 (1 : 500) (Cat # MAB1080). Proteins were visualized using a goat anti - mouse secondary antibody conjugated to HRP and an ECL detection system.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometry analysis of Jurkat cells, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line). -
Gene Info — PIN1
Entrez GeneID
5300Protein Accession#
NP_006212Gene Name
PIN1
Gene Alias
DOD, UBL5
Gene Description
peptidylprolyl cis/trans isomerase, NIMA-interacting 1
Omim ID
601052Gene Ontology
HyperlinkGene Summary
The human PIN1 gene encodes an essential nuclear peptidylprolyl cis-trans isomerase (PPIase; EC 5.2.1.8) involved in regulation of mitosis. PIN1 belongs to a class of PPIases that includes the E. coli parvulin, yeast Ess1, and Drosophila dodo (dod) gene products (Lu et al., 1996 [PubMed 8606777]).[supplied by OMIM
Other Designations
peptidyl-prolyl cis/trans isomerase, NIMA-interacting|prolyl isomerase|protein (peptidyl-prolyl cis/trans isomerase) NIMA-interacting 1|protein (peptidylprolyl cis/trans isomerase) NIMA-interacting 1
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Interactome
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Disease
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Publication Reference
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Analysis of binding interfaces of the human scaffold protein AXIN1 by peptide microarrays.
Harnoš J, Ryneš J, Víšková P, Trantírková SF, Ešner LB, Trantírek L, Bryja V.
The Journal of Biological Chemistry 2018 Oct; 293(42):16337.
Application：Peptide array, Recombinant protein.
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Pin1: a therapeutic target in Alzheimer neurodegeneration.
Hamdane M, Smet C, Sambo AV, Leroy A, Wieruszeski JM, Delobel P, Maurage CA, Ghestem A, Wintjens R, Begard S, Sergeant N, Delacourte A, Horvath D, Landrieu I, Lippens G, Buee L.
Journal of Molecular Neuroscience 2002 Dec; 19(3):275.
Application：WB-Ce, WB-Ti, Human, SY5Y cells, Brain.
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Role of Pin1 in the regulation of p53 stability and p21 transactivation, and cell cycle checkpoints in response to DNA damage.
Wulf GM, Liou YC, Ryo A, Lee SW, Lu KP.
The Journal of Biological Chemistry 2002 Dec; 277(50):47976.
Application：WB-Ce, Human, Mouse, T47D cells, MEFs.
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The prolyl isomerase Pin1 reveals a mechanism to control p53 functions after genotoxic insults.
Zacchi P, Gostissa M, Uchida T, Salvagno C, Avolio F, Volinia S, Ronai Z, Blandino G, Schneider C, Del Sal G.
Nature 2002 Oct; 419(6909):853.
Application：IP-Wb, WB-Tr, Human, Mouse, 293 cells, MEFs.
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Analysis of binding interfaces of the human scaffold protein AXIN1 by peptide microarrays.
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