PPP2CA recombinant monoclonal antibody, clone R04-4G4
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More Files
- More Functions
Western Blot
Western blot analysis of Lane 1: rat brain, Lane 2: C6, Lane 3: CHO-K1 and Lane 4: Hela lysates with PPP2CA recombinant monoclonal antibody, clone R04-4G4 (Cat # RAB01987).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin/PFA-fixed paraffin-embedded sections) of human lung cancer with PPP2CA recombinant monoclonal antibody, clone R04-4G4 (Cat # RAB01987).
High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human PPP2CA.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human PPP2CA.
Theoretical MW (kDa)
Calculated MW: 36 kD
Reactivity
Hamster, Human, Mouse, Rat
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1:100)
Immunoprecipitation (1:20)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at -20 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: rat brain, Lane 2: C6, Lane 3: CHO-K1 and Lane 4: Hela lysates with PPP2CA recombinant monoclonal antibody, clone R04-4G4 (Cat # RAB01987).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin/PFA-fixed paraffin-embedded sections) of human lung cancer with PPP2CA recombinant monoclonal antibody, clone R04-4G4 (Cat # RAB01987).
High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.Immunohistochemistry (Frozen sections)
Immunofluorescence
Immunoprecipitation
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Gene Info — PPP2CA
Entrez GeneID
5515Protein Accession#
P67775Gene Name
PPP2CA
Gene Alias
PP2Ac, PP2CA, RP-C
Gene Description
protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform
Omim ID
176915Gene Ontology
HyperlinkGene Summary
This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit. [provided by RefSeq
Other Designations
PP2A-alpha|protein phosphatase 2, catalytic subunit, alpha isoform|replication protein C|serine/threonine protein phosphatase 2A, catalytic subunit, alpha isoform|serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform
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Interactome
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Pathway
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Disease
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