PDHA1 recombinant monoclonal antibody, clone R06-1J4
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More Files
- More Functions
Western Blot
Western blot analysis of Lane 1: C6, Lane 2: 3T3 lysates with PDHA1 recombinant monoclonal antibody, clone R06-1J4 (Cat # RAB01970).
Immunocytochemistry
Immunocytochemical staining of Hela with PDHA1 recombinant monoclonal antibody, clone R06-1J4 (Cat # RAB01970).(A) PDHA1 (green) and (B) DAPI (blue).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human PDHA1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human PDHA1.
Theoretical MW (kDa)
Calculated MW: 43 kD
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunocytochemistry
Immunofluorescence (1:50-1:200)
Immunoprecipitation (1:20)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at -20 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: C6, Lane 2: 3T3 lysates with PDHA1 recombinant monoclonal antibody, clone R06-1J4 (Cat # RAB01970).Immunocytochemistry
Immunocytochemical staining of Hela with PDHA1 recombinant monoclonal antibody, clone R06-1J4 (Cat # RAB01970).(A) PDHA1 (green) and (B) DAPI (blue).Immunofluorescence
Immunoprecipitation
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Gene Info — PDHA1
Entrez GeneID
5160Protein Accession#
P08559Gene Name
PDHA1
Gene Alias
PDHA, PDHCE1A, PHE1A
Gene Description
pyruvate dehydrogenase (lipoamide) alpha 1
Gene Ontology
HyperlinkGene Summary
The pyruvate dehydrogenase complex is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA. The PDH complex is composed of multiple copies of 3 enzymes: E1 (PDHA1); dihydrolipoyl transacetylase (DLAT; MIM 608770) (E2; EC 2.3.1.12); and dihydrolipoyl dehydrogenase (DLD; MIM 238331) (E3; EC 1.8.1.4). The E1 enzyme is a heterotetramer of 2 alpha and 2 beta subunits. The E1-alpha subunit contains the E1 active site and plays a key role in the function of the PDH complex (Brown et al., 1994 [PubMed 7853374]).[supplied by OMIM
Other Designations
OTTHUMP00000023015|pyruvate dehydrogenase E1 alpha subunit
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Interactome
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Pathway
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Butanoate metabolism
- Citrate cycle (TCA cycle)
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