PDHA1 (phospho S293) polyclonal antibody (DyLight 650)
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of PDHA1.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding S293 human PDHA1.
Host
Rabbit
Reactivity
Human
Specificity
This is specific for the phosphorylated Serine 293 form of the PDHE1 alpha protein. It is recommended that BSA rather than non-fat milk be used in membrane blocking and antibody dilution. This antibody is useful for Immunocytochemistry/immunofluorescence and Western blot, where a band is seen ~43 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Form
Liquid
Conjugation
DyLight 650
Purification
Immunogen affinity purification
Recommend Usage
Immunocytochemistry/Immunofluorescence
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM sodium borate (0.05% sodium azide).
Storage Instruction
Store at 4°C in the dark.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunocytochemistry
Immunofluorescence
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Gene Info — PDHA1
Entrez GeneID
5160Protein Accession#
P08559Gene Name
PDHA1
Gene Alias
PDHA, PDHCE1A, PHE1A
Gene Description
pyruvate dehydrogenase (lipoamide) alpha 1
Gene Ontology
HyperlinkGene Summary
The pyruvate dehydrogenase complex is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA. The PDH complex is composed of multiple copies of 3 enzymes: E1 (PDHA1); dihydrolipoyl transacetylase (DLAT; MIM 608770) (E2; EC 2.3.1.12); and dihydrolipoyl dehydrogenase (DLD; MIM 238331) (E3; EC 1.8.1.4). The E1 enzyme is a heterotetramer of 2 alpha and 2 beta subunits. The E1-alpha subunit contains the E1 active site and plays a key role in the function of the PDH complex (Brown et al., 1994 [PubMed 7853374]).[supplied by OMIM
Other Designations
OTTHUMP00000023015|pyruvate dehydrogenase E1 alpha subunit
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Interactome
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Pathway
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Butanoate metabolism
- Citrate cycle (TCA cycle)
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