ATG3 recombinant monoclonal antibody, clone R06-9E7
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More Files
- More Functions
Western Blot
Western blot analysis of ATG3 in K562, rat Brain, C6, 3T3, Hela lysates using human ATG3 recombinant monoclonal antibody, clone R06-9E7 (Cat # RAB01581).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Formalin-fixed paraffin-embedded sections) of Human tonsil with ATG3 recombinant monoclonal antibody, clone R06-9E7 (Cat # RAB01581).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against synthetic peptide of human ATG3.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human ATG3
Theoretical MW (kDa)
Calculated MW: 36 kD
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence(1:50-1:200)
Immunohistochemistry (1:50-1:100)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50mM Tris-Glycine, pH 7.4, (0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C for short term. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of ATG3 in K562, rat Brain, C6, 3T3, Hela lysates using human ATG3 recombinant monoclonal antibody, clone R06-9E7 (Cat # RAB01581).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Formalin-fixed paraffin-embedded sections) of Human tonsil with ATG3 recombinant monoclonal antibody, clone R06-9E7 (Cat # RAB01581).Immunohistochemistry (Frozen sections)
Immunocytochemistry
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Gene Info — ATG3
Entrez GeneID
64422Protein Accession#
Q9NT62Gene Name
ATG3
Gene Alias
APG3, APG3-LIKE, APG3L, DKFZp564M1178, FLJ22125, MGC15201, PC3-96
Gene Description
ATG3 autophagy related 3 homolog (S. cerevisiae)
Omim ID
609606Gene Ontology
HyperlinkGene Summary
Autophagy is a process of bulk degradation of cytoplasmic components by the lysosome or vacuole. Human ATG3 displays the same enzymatic characteristics in vitro as yeast Apg3, a protein-conjugating enzyme essential for autophagy (Tanida et al., 2002 [PubMed 11825910]).[supplied by OMIM
Other Designations
2610016C12Rik|APG3 autophagy 3-like|Apg3p|autophagy Apg3p/Aut1p-like
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Interactome
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Pathway
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Disease
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