CLDN3 recombinant monoclonal antibody, clone R08-4F8
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More Files
- More Functions
Western Blot
Western blot analysis of Claudin 3 in MCF-7 lysates using human Claudin 3 recombinant monoclonal antibody, clone R08-4F8 (Cat # RAB01500).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against synthetic peptide of human Claudin 3.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human Claudin 3
Theoretical MW (kDa)
Calculated MW: 23 kD
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence (1:50-1:200)
Immunoprecipitation (1:20)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C for short term. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Claudin 3 in MCF-7 lysates using human Claudin 3 recombinant monoclonal antibody, clone R08-4F8 (Cat # RAB01500).Immunocytochemistry
Immunoprecipitation
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Gene Info — CLDN3
Entrez GeneID
1365Protein Accession#
O15551Gene Name
CLDN3
Gene Alias
C7orf1, CPE-R2, CPETR2, HRVP1, RVP1
Gene Description
claudin 3
Omim ID
602910Gene Ontology
HyperlinkGene Summary
Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this intronless gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. It is also a low-affinity receptor for Clostridium perfringens enterotoxin, and shares aa sequence similarity with a putative apoptosis-related protein found in rat. [provided by RefSeq
Other Designations
CPE-receptor 2|Clostridium perfringens enterotoxin receptor 2|OTTHUMP00000025042|OTTHUMP00000160506|claudin-3|ventral prostate.1-like protein
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Interactome
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Pathway
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