TUBA4A recombinant monoclonal antibody, clone R02-2H3
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human TUBA4A.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein corresponding to human alpha Tubulin.
Theoretical MW (kDa)
Calculated MW: 50 kD
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunocytochemistry
Immunofluorescence
Immunohistochemistry (Frozen sections)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoprecipitation
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C. For longer storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of alpha Tubulin in Hela, CHO-K1, rat Brain lysates using alpha Tubulin antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry analysis of paraffin-embedded Human tonsil using alpha Tubulin antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.Immunohistochemistry (Frozen sections)
Immunocytochemistry
Immunofluorescence
Immunoprecipitation
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Gene Info — TUBA4A
Entrez GeneID
7277Protein Accession#
P68366Gene Name
TUBA4A
Gene Alias
FLJ30169, H2-ALPHA, TUBA1
Gene Description
tubulin, alpha 4a
Omim ID
191110Gene Ontology
HyperlinkGene Summary
Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. This gene encodes an alpha tubulin that is a highly conserved homolog of a rat testis-specific alpha tubulin. [provided by RefSeq
Other Designations
tubulin alpha-1 chain|tubulin, alpha 1 (testis specific)
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Interactome
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Pathway
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Disease
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