MCM5 recombinant monoclonal antibody, clone R05-5E5
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human MCM5.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein corresponding to human MCM5.
Theoretical MW (kDa)
Calculated MW: 82 kD
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (Frozen sections)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunocytochemistry
Immunofluorescence
Immunoprecipitation
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C. For longer storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of MCM5 in Jurkat, C6, Hela lysates using MCM5 antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Frozen sections)
Immunocytochemistry
Immunocytochemistry analysis of MCM5(green) in Hela using MCM5 antibody,and DAPI(blue).Immunofluorescence
Immunoprecipitation
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Gene Info — MCM5
Entrez GeneID
4174Protein Accession#
P33992Gene Name
MCM5
Gene Alias
CDC46, MGC5315, P1-CDC46
Gene Description
minichromosome maintenance complex component 5
Omim ID
602696Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is structurally very similar to the CDC46 protein from S. cerevisiae, a protein involved in the initiation of DNA replication. The encoded protein is a member of the MCM family of chromatin-binding proteins and can interact with at least two other members of this family. The encoded protein is upregulated in the transition from the G0 to G1/S phase of the cell cycle and may actively participate in cell cycle regulation. [provided by RefSeq
Other Designations
DNA replication licensing factor|MCM5 minichromosome maintenance deficient 5, cell division cycle 46|minichromosome maintenance deficient 5 (cell division cycle 46)
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Interactome
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Disease
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