MAP3K5 (phospho S83) polyclonal antibody
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More Files
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Western Blot
Immunoblot of MAP3K5 (phospho S83) polyclonal antibody (Cat # PAB9947) shows specificity for phosphorylated human MAP3K5. MAP3K5 (phospho S83) polyclonal antibody was tested by immunoblot against COS-7 cell lysates after transient transfection with (Lane 1) vector only, (Lane 2) recombinant HA-MAP3K5, and (Lane 3) recombinant human HA-MA3K5 where S83 was substituted with an alanine residue.
A : anti-HA.
B : MAP3K5 (phospho S83) polyclonal antibody blocked with non-phospho peptide.
C : MAP3K5 (phospho S83) polyclonal antibody blocked with phospho peptide.
A 155 kDa band corresponding to human MAP3K5 is detected.
Enzyme-linked Immunoabsorbent Assay
ELISA results of MAP3K5 (phospho S83) polyclonal antibody (Cat # PAB9947) tested against BSA conjugates of non-phospho and phospho forms of immunizing peptide.
Each well was coated with 0.1 mg of conjugate.
The starting dilution of antibody was 1 : 1,000 and each point on the X-axis represents a 2-fold dilution.
HRP conjugated Goat-anti-Rabbit IgG H&L and TMB substrate were used for detection.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphpeptide of MAP3K5.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S83 of human MAP3K5.
Host
Rabbit
Reactivity
Human, Mouse
Specificity
This phospho specific polyclonal antibody reacts human pS83 ASK1.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:5000-1:10000)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunoblot of MAP3K5 (phospho S83) polyclonal antibody (Cat # PAB9947) shows specificity for phosphorylated human MAP3K5. MAP3K5 (phospho S83) polyclonal antibody was tested by immunoblot against COS-7 cell lysates after transient transfection with (Lane 1) vector only, (Lane 2) recombinant HA-MAP3K5, and (Lane 3) recombinant human HA-MA3K5 where S83 was substituted with an alanine residue.
A : anti-HA.
B : MAP3K5 (phospho S83) polyclonal antibody blocked with non-phospho peptide.
C : MAP3K5 (phospho S83) polyclonal antibody blocked with phospho peptide.
A 155 kDa band corresponding to human MAP3K5 is detected.Enzyme-linked Immunoabsorbent Assay
ELISA results of MAP3K5 (phospho S83) polyclonal antibody (Cat # PAB9947) tested against BSA conjugates of non-phospho and phospho forms of immunizing peptide.
Each well was coated with 0.1 mg of conjugate.
The starting dilution of antibody was 1 : 1,000 and each point on the X-axis represents a 2-fold dilution.
HRP conjugated Goat-anti-Rabbit IgG H&L and TMB substrate were used for detection. -
Gene Info — MAP3K5
Entrez GeneID
4217Gene Name
MAP3K5
Gene Alias
ASK1, MAPKKK5, MEKK5
Gene Description
mitogen-activated protein kinase kinase kinase 5
Omim ID
602448Gene Ontology
HyperlinkGene Summary
Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK. [provided by RefSeq
Other Designations
MAP/ERK kinase kinase 5|MAPK/ERK kinase kinase 5|OTTHUMP00000017275|apoptosis signal regulating kinase
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Interactome
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Pathway
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Disease
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Publication Reference
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Negative feedback regulation of ASK1 by protein phosphatase 5 (PP5) in response to oxidative stress.
Morita K, Saitoh M, Tobiume K, Matsuura H, Enomoto S, Nishitoh H, Ichijo H.
The EMBO Journal 2001 Nov; 20(21):6028.
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The cell cycle-regulatory CDC25A phosphatase inhibits apoptosis signal-regulating kinase 1.
Zou X, Tsutsui T, Ray D, Blomquist JF, Ichijo H, Ucker DS, Kiyokawa H.
Mol Cell Biol 2001 Jul; 21(14):4818.
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Molecular mechanisms of the decision between life and death: regulation of apoptosis by apoptosis signal-regulating kinase 1.
Matsuzawa A, Ichijo H.
Journal of Biochemistry 2001 Jul; 130(1):1.
Application：IHC, WB-Tr, Human, Cancers, Mammalian cells.
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Negative feedback regulation of ASK1 by protein phosphatase 5 (PP5) in response to oxidative stress.
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