RAF1 polyclonal antibody
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More Files
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of RAF1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to amino acids 637-648 of human RAF1.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This sequence is conserved in C-Rafs from many species, and is not found in A-Raf or B-Raf.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:2000)
Western Blot (1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1 mg/mL BSA, 0.05% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Enzyme-linked Immunoabsorbent Assay
Western Blot (Recombinant protein)
Western blot of GST fusion protein containing human RAF1. The blot was probed with RAF1 polyclonal antibody (Cat # PAB7870) in the presence (lane 2) or absence (lane 1) of RAF1 (C-terminus) blocking peptide (lane 2). -
Gene Info — RAF1
Entrez GeneID
5894Gene Name
RAF1
Gene Alias
CRAF, NS5, Raf-1, c-Raf
Gene Description
v-raf-1 murine leukemia viral oncogene homolog 1
Gene Ontology
HyperlinkGene Summary
This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2. [provided by RefSeq
Other Designations
Oncogene RAF1|raf proto-oncogene serine/threonine protein kinase
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Interactome
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Pathway
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Disease
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Publication Reference
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Identification of Raf-1 S471 as a novel phosphorylation site critical for Raf-1 and B-Raf kinase activities and for MEK binding.
Zhu J, Balan V, Bronisz A, Balan K, Sun H, Leicht DT, Luo Z, Qin J, Avruch J, Tzivion G.
Molecular Biology of the Cell 2005 Oct; 16(10):4733.
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Serine and tyrosine phosphorylations cooperate in Raf-1, but not B-Raf activation.
Mason CS, Springer CJ, Cooper RG, Superti-Furga G, Marshall CJ, Marais R.
The EMBO Journal 1999 Apr; 18(8):2137.
Application:IP, KA, WB-Ce, WB-Tr, Monkey, Mouse, COS, NIH/3T3 cells.
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Identification of Raf-1 S471 as a novel phosphorylation site critical for Raf-1 and B-Raf kinase activities and for MEK binding.
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