CBX8 polyclonal antibody
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More Files
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of CBX8.
Immunogen
A synthetic peptide corresponding to human CBX8.
Sequence
C-RERGTSRVDDKPSS
Host
Goat
Theoretical MW (kDa)
43.4
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Western Blot (0.1-0.3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
CBX8 polyclonal antibody (Cat # PAB7489) (0.1 ug/mL) staining of K-562 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — CBX8
Entrez GeneID
57332Protein Accession#
NP_065700.1Gene Name
CBX8
Gene Alias
HPC3, PC3, RC1
Gene Description
chromobox homolog 8 (Pc class homolog, Drosophila)
Gene Ontology
HyperlinkGene Summary
O
Other Designations
chromobox homolog 8|polycomb 3
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Interactome
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Publication Reference
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Bypass of senescence by the polycomb group protein CBX8 through direct binding to the INK4A-ARF locus.
Dietrich N, Bracken AP, Trinh E, Schjerling CK, Koseki H, Rappsilber J, Helin K, Hansen KH.
The EMBO Journal 2007 Mar; 26(6):1637.
Application:ChIP, IF, WB, Human, Human diploid fibroblasts, U2OS cells.
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Bypass of senescence by the polycomb group protein CBX8 through direct binding to the INK4A-ARF locus.
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