DDX19A/DDX19B polyclonal antibody
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More Files
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Western Blot (Cell lysate)
The DDX19A/DDX19B polyclonal antibody (Cat # PAB7319) (0.5 ug/mL) staining of K-562 cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of DDX19A/DDX19B.
Immunogen
A synthetic peptide corresponding to human DDX19A/DDX19B.
Sequence
C-DLPVDKDGNPDNETY
Host
Goat
Theoretical MW (kDa)
53.9, 50.5, 41.8, 54
Reactivity
Human
Specificity
This antibody is expected to recognize DDX19A (NP_060802.1) and all reported isoforms of DDX19B (NP_009173.1, NP_001014451.1 and NP_001014449.1).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:4000)
Western Blot (0.5-1.5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
The DDX19A/DDX19B polyclonal antibody (Cat # PAB7319) (0.5 ug/mL) staining of K-562 cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — DDX19B
Entrez GeneID
11269Protein Accession#
NP_009173.1 (Gene ID : 11269);NP_060802.1 (Gene ID : 55308);NP_001014451.1 (Gene ID : 11269);NP_001014451.1 (Gene ID : 11269);NP_001014449.1 (Gene ID : 11269)Gene Name
DDX19B
Gene Alias
DBP5, DDX19, RNAh
Gene Description
DEAD (Asp-Glu-Ala-As) box polypeptide 19B
Omim ID
605812Gene Ontology
HyperlinkGene Summary
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which exhibits RNA-dependent ATPase and ATP-dependent RNA-unwinding activities. This protein is recruited to the cytoplasmic fibrils of the nuclear pore complex, where it participates in the export of mRNA from the nucleus. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
ATP-dependent RNA helicase DDX19|DEAD (Asp-Glu-Ala-As) box polypeptide 19|DEAD-box RNA helicase DEAD5|DEAD-box protein 5|DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 19 (Dbp5, yeast, homolog)|yeast Dbp5 homolog
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Gene Info — DDX19A
Entrez GeneID
55308Protein Accession#
NP_009173.1 (Gene ID : 11269);NP_060802.1 (Gene ID : 55308);NP_001014451.1 (Gene ID : 11269);NP_001014451.1 (Gene ID : 11269);NP_001014449.1 (Gene ID : 11269)Gene Name
DDX19A
Gene Alias
DDX19-DDX19L, DDX19L, DKFZp686C21137, FLJ11126
Gene Description
DEAD (Asp-Glu-Ala-As) box polypeptide 19A
Gene Ontology
HyperlinkOther Designations
DDX19-like protein|DEAD (Asp-Glu-Ala-As) box polypeptide 19-like|RNA helicase
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Interactome
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Publication Reference
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Transport of messenger RNA from the nucleus to the cytoplasm.
Cole CN, Scarcelli JJ.
Current Opinion in Cell Biology 2006 Jun; 18(3):299.
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Transport of messenger RNA from the nucleus to the cytoplasm.
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