VPS37C polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of VPS37C.
Immunogen
A synthetic peptide corresponding to internal region of human VPS37C.
Sequence
C-ETLKDKTLQELEELQ
Host
Goat
Theoretical MW (kDa)
38.7
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:32000)
Western Blot (0.03-0.1 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (3-6 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
VPS37C polyclonal antibody (Cat # PAB6825) (0.03g/mL) staining of human duodenum lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
VPS37C polyclonal antibody (Cat # PAB6825)(3.8 ug/mL) staining of paraffin embedded human prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — VPS37C
Entrez GeneID
55048Protein Accession#
NP_060436.4Gene Name
VPS37C
Gene Alias
FLJ20847
Gene Description
vacuolar protein sorting 37 homolog C (S. cerevisiae)
Omim ID
610038Gene Ontology
HyperlinkGene Summary
VPS37C is a subunit of ESCRT-I (endosomal sorting complex required for transport I), a complex in the class E vacuolar protein sorting (VPS) pathway required for sorting ubiquitinated transmembrane proteins into internal vesicles of multivesicular bodies (Eastman et al., 2005 [PubMed 15509564]).[supplied by OMIM
Other Designations
vacuolar protein sorting 37C
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Interactome
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Pathway
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Disease
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Publication Reference
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Identification of human VPS37C, a component of endosomal sorting complex required for transport-I important for viral budding.
Eastman SW, Martin-Serrano J, Chung W, Zang T, Bieniasz PD.
The Journal of Biological Chemistry 2005 Jan; 280(1):628.
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Identification of human VPS37C, a component of endosomal sorting complex required for transport-I important for viral budding.
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