SH2D1A polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Tissue lysate)
SH2D1A polyclonal antibody (Cat # PAB6819) staining (0.3 ug/mL) of human thymus lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Western Blot (Transfected lysate)
HEK293 overexpressing SH2D1A and probed with SH2D1A polyclonal antibody (Cat # PAB6819) (mock transfection in first lane), tested by Origene.
-
Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of SH2D1A.
Immunogen
A synthetic peptide corresponding to human SH2D1A.
Sequence
C-HKRYFRKIKN
Host
Goat
Theoretical MW (kDa)
14.2, 13.9
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:16000)
Western Blot (0.3-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Tissue lysate)
SH2D1A polyclonal antibody (Cat # PAB6819) staining (0.3 ug/mL) of human thymus lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Western Blot (Transfected lysate)
HEK293 overexpressing SH2D1A and probed with SH2D1A polyclonal antibody (Cat # PAB6819) (mock transfection in first lane), tested by Origene.Enzyme-linked Immunoabsorbent Assay
-
Gene Info — SH2D1A
Entrez GeneID
4068Protein Accession#
NP_002342.1Gene Name
SH2D1A
Gene Alias
DSHP, EBVS, FLJ18687, FLJ92177, IMD5, LYP, MTCP1, SAP, XLP, XLPD
Gene Description
SH2 domain protein 1A
Gene Ontology
HyperlinkGene Summary
This gene encodes a protein that plays a major role in the bidirectional stimulation of T and B cells. This protein contains an SH2 domain and a short tail. It associates with the signaling lymphocyte-activation molecule, thereby acting as an inhibitor of this transmembrane protein by blocking the recruitment of the SH2-domain-containing signal-transduction molecule SHP-2 to its docking site. This protein can also bind to other related surface molecules that are expressed on activated T, B and NK cells, thereby modifying signal transduction pathways in these cells. Mutations in this gene cause lymphoproliferative syndrome X-linked type 1 or Duncan disease, a rare immunodeficiency characterized by extreme susceptibility to infection with Epstein-Barr virus, with symptoms including severe mononucleosis and malignant lymphoma. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
Duncan's disease|OTTHUMP00000023976|SLAM-associated protein|T cell signal transduction molecule SAP|signaling lymphocyte activation molecule-associated protein
-
Interactome
-
Pathway
-
Disease
-
Publication Reference
-
The adaptor protein SH2D1A regulates signaling through CD150 (SLAM) in B cells.
Mikhalap SV, Shlapatska LM, Yurchenko OV, Yurchenko MY, Berdova GG, Nichols KE, Clark EA, Sidorenko SP.
Blood 2004 Dec; 104(13):4063.
Application：IHC-P, WB, Human, Primary lymphoma cells, Tonsils.
-
The adaptor protein SH2D1A regulates signaling through CD150 (SLAM) in B cells.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com