ADAM12 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
ADAM12 polyclonal antibody (Cat # PAB6697) staining (0.1 ug/mL) of human heart lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of ADAM12.
Immunogen
A synthetic peptide corresponding to human ADAM12.
Sequence
AARPLPVSPARALC
Host
Goat
Theoretical MW (kDa)
99.6, 80.5
Reactivity
Human
Specificity
This antibody is expected to recognize both the longer membrane-bound form of human ADAM12 (NP_003465) and the shorter soluble ADAM12 splice isoform (NP_067673). This antibody does not cross-react with other ADAMS.
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:64000)
Western blot (0.1-0.3 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (3-5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
ADAM12 polyclonal antibody (Cat # PAB6697) staining (0.1 ug/mL) of human heart lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Immunohistochemistry
Enzyme-linked Immunoabsorbent Assay
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Gene Info — ADAM12
Entrez GeneID
8038Protein Accession#
NP_003465;NP_067673Gene Name
ADAM12
Gene Alias
MCMP, MCMPMltna, MLTN, MLTNA
Gene Description
ADAM metallopeptidase domain 12
Omim ID
602714Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the ADAM (a disintegrin and metalloprotease) protein family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. This gene has two alternatively spliced transcripts: a shorter secreted form and a longer membrane-bound form. The shorter form is found to stimulate myogenesis. [provided by RefSeq
Other Designations
OTTHUMP00000020727|OTTHUMP00000046766|a disintegrin and metalloproteinase domain 12 (meltrin alpha)|meltrin alpha
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Interactome
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Disease
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Publication Reference
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Binding of ADAM12, a marker of skeletal muscle regeneration, to the muscle-specific actin-binding protein, alpha -actinin-2, is required for myoblast fusion.
Galliano MF, Huet C, Frygelius J, Polgren A, Wewer UM, Engvall E.
The Journal of Biological Chemistry 2000 May; 275(18):13933.
Application：IP, WB-Ce, WB-Tr, Mouse, C2C12, CHO Cells.
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Binding of ADAM12, a marker of skeletal muscle regeneration, to the muscle-specific actin-binding protein, alpha -actinin-2, is required for myoblast fusion.
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