ATP6IP2 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
ATP6IP2 polyclonal antibody (Cat # PAB6489) staining (0.5 ug/mL) of human kidney lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Flow Cytometry
ATP6IP2 polyclonal antibody (Cat # PAB6489) Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of ATP6IP2.
Immunogen
A synthetic peptide corresponding to C-terminus of human ATP6IP2.
Sequence
C-SIIYRMTNQKIRMD
Host
Goat
Theoretical MW (kDa)
39
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Antigen affinity purification
Recommend Usage
ELISA (1:8000)
Flow Cytometry (10 ug/mL)
Western Blot (0.3-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
ATP6IP2 polyclonal antibody (Cat # PAB6489) staining (0.5 ug/mL) of human kidney lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Immunohistochemistry
Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
ATP6IP2 polyclonal antibody (Cat # PAB6489) Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody. -
Gene Info — ATP6AP2
Entrez GeneID
10159Protein Accession#
NP_005756.2Gene Name
ATP6AP2
Gene Alias
APT6M8-9, ATP6IP2, ATP6M8-9, ELDF10, HT028, M8-9, MGC99577, MRXE, MSTP009, XMRE
Gene Description
ATPase, H+ transporting, lysosomal accessory protein 2
Gene Ontology
HyperlinkGene Summary
This gene encodes a protein that is associated with adenosine triphosphatases (ATPases). Proton-translocating ATPases have fundamental roles in energy conservation, secondary active transport, acidification of intracellular compartments, and cellular pH homeostasis. There are three classes of ATPases- F, P, and V. The vacuolar (V-type) ATPases have a transmembrane proton-conducting sector and an extramembrane catalytic sector. The encoded protein has been found associated with the transmembrane sector of the V-type ATPases. [provided by RefSeq
Other Designations
ATPase, H+ transporting, lysosomal (vacuolar proton pump) membrane sector associated protein M8-9|ATPase, H+ transporting, lysosomal interacting protein 2|OTTHUMP00000025771|V-ATPase M8.9 subunit|embryonic liver differentiation factor 10|renin receptor|va
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Interactome
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Disease
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Publication Reference
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Identification and characterization of a novel 9.2-kDa membrane sector-associated protein of vacuolar proton-ATPase from chromaffin granules.
Ludwig J, Kerscher S, Brandt U, Pfeiffer K, Getlawi F, Apps DK, Schagger H.
The Journal of Biological Chemistry 1998 May; 273(18):10939.
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Identification and characterization of a novel 9.2-kDa membrane sector-associated protein of vacuolar proton-ATPase from chromaffin granules.
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