TRIM5 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
TRIM5 polyclonal antibody (Cat # PAB6383) staining (1 ug/mL) of human brain lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
TRIM5 polyclonal antibody (Cat # PAB6383) staining (1 ug/mL) of paraffin embedded human tonsil. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.A) positive cells next to a secondary follicle, B) positive cells below tonsillar epithelium.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of TRIM5.
Immunogen
A synthetic peptide corresponding to human TRIM5.
Sequence
ASGILVNVKEEVTC
Host
Goat
Theoretical MW (kDa)
56.3, 40.1, 37.7
Reactivity
Human
Specificity
This antibody is expected to recognize all three human isoforms of this protein.
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:16000)
Western Blot (1-3 ug/mL)
Immunohistochemistry (1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
TRIM5 polyclonal antibody (Cat # PAB6383) staining (1 ug/mL) of human brain lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
TRIM5 polyclonal antibody (Cat # PAB6383) staining (1 ug/mL) of paraffin embedded human tonsil. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.A) positive cells next to a secondary follicle, B) positive cells below tonsillar epithelium.Enzyme-linked Immunoabsorbent Assay
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Gene Info — TRIM5
Entrez GeneID
85363Protein Accession#
NP_149023.1;NP_149083.1;NP_149084.1Gene Name
TRIM5
Gene Alias
RNF88, TRIM5alpha
Gene Description
tripartite motif-containing 5
Omim ID
608487Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic bodies. Its function has not been identified. Five alternatively spliced transcript variants for this gene have been described. However, the full length nature of two of the variants has not been determined. [provided by RefSeq
Other Designations
OTTHUMP00000069811|OTTHUMP00000069812|tripartite motif protein TRIM|tripartite motif protein TRIM5|tripartite motif-containing protein 5 alpha
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Interactome
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Disease
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Publication Reference
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Restriction of foamy viruses by primate Trim5alpha.
Yap MW, Lindemann D, Stanke N, Reh J, Westphal D, Hanenberg H, Ohkura S, Stoye JP.
Journal of Virology 2008 Jun; 82(11):5429.
Application：WB, Human, HT1080 cells.
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The design of artificial retroviral restriction factors.
Yap MW, Mortuza GB, Taylor IA, Stoye JP.
Virology 2007 Sep; 365(2):302.
Application：Flow Cyt, Human, HT1080 cells.
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All three variable regions of the TRIM5alpha B30.2 domain can contribute to the specificity of retrovirus restriction.
Ohkura S, Yap MW, Sheldon T, Stoye JP.
Journal of Virology 2006 Sep; 80(17):8554.
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The tripartite motif family identifies cell compartments.
Reymond A, Meroni G, Fantozzi A, Merla G, Cairo S, Luzi L, Riganelli D, Zanaria E, Messali S, Cainarca S, Guffanti A, Minucci S, Pelicci PG, Ballabio A.
The EMBO Journal 2001 May; 20(9):2140.
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Restriction of foamy viruses by primate Trim5alpha.
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