DCXR polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of DCXR.
Immunogen
A synthetic peptide corresponding to human DCXR.
Sequence
GSTLPVEGGFWAC
Host
Goat
Theoretical MW (kDa)
25.9
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Western Blot (0.1-0.3 ug/mL)
Immunohistochemistry (1-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
DCXR polyclonal antibody (Cat # PAB6204) staining (0.1 ug/mL) of human kidney lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
DCXR polyclonal antibody (Cat # PAB6204) staining (1 ug/mL) of paraffin embedded human kidney. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — DCXR
Entrez GeneID
51181Protein Accession#
NP_057370.1Gene Name
DCXR
Gene Alias
DCR, HCR2, HCRII, KIDCR, P34H, SDR20C1
Gene Description
dicarbonyl/L-xylulose reductase
Omim ID
608347Gene Ontology
HyperlinkGene Summary
DCXR is an enzyme that has both diacetyl reductase (EC 1.1.1.5) and L-xylulose reductase (EC 1.1.1.10) activities (Nakagawa et al., 2002 [PubMed 11882650]).[supplied by OMIM
Other Designations
carbonyl reductase II|human carbonyl reductase 2|kidney dicarbonyl reductase|short chain dehydrogenase/reductase family 20C, member 1
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Interactome
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Pathway
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Publication Reference
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Molecular characterization of mammalian dicarbonyl/L-xylulose reductase and its localization in kidney.
Nakagawa J, Ishikura S, Asami J, Isaji T, Usami N, Hara A, Sakurai T, Tsuritani K, Oda K, Takahashi M, Yoshimoto M, Otsuka N, Kitamura K.
The Journal of Biological Chemistry 2002 May; 277(20):17883.
Application:IHC, WB-Ti, Mouse, Rat, Liver, Kidney.
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Molecular characterization of mammalian dicarbonyl/L-xylulose reductase and its localization in kidney.
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