STAP2 polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
STAP2 polyclonal antibody (Cat # PAB6042) staining (0.03 ug/mL) of human heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
STAP2 polyclonal antibody (Cat # PAB6042)(3.8 ug/mL) staining of paraffin embedded human cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of STAP2.
Immunogen
A synthetic peptide corresponding to C-terminus of human STAP2.
Sequence
C-ELQKKLEKRRALEH
Host
Goat
Theoretical MW (kDa)
49.6
Reactivity
Human
Specificity
This antibody is expected to recognize both reported isoforms (as represented by NP_060190.2 and NP_001013863.1).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:16000)
Western Blot (0.05-0.3 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
STAP2 polyclonal antibody (Cat # PAB6042) staining (0.03 ug/mL) of human heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
STAP2 polyclonal antibody (Cat # PAB6042)(3.8 ug/mL) staining of paraffin embedded human cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — STAP2
Entrez GeneID
55620Protein Accession#
NP_060190.2;NP_001013863.1Gene Name
STAP2
Gene Alias
BKS, FLJ20234
Gene Description
signal transducing adaptor family member 2
Omim ID
607881Gene Ontology
HyperlinkGene Summary
This gene encodes the substrate of breast tumor kinase, an Src-type non-receptor tyrosine kinase. The encoded protein possesses domains and several tyrosine phosphorylation sites characteristic of adaptor proteins that mediate the interactions linking proteins involved in signal transduction pathways. Alternative splicing results in multiple transcript variants. [provided by RefSeq
Other Designations
breast tumor kinase substrate|brk kinase substrate|signal-transducing adaptor protein-2
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Disease
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Publication Reference
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STAP-2/BKS, an adaptor/docking protein, modulates STAT3 activation in acute-phase response through its YXXQ motif.
Minoguchi M, Minoguchi S, Aki D, Joo A, Yamamoto T, Yumioka T, Matsuda T, Yoshimura A.
The Journal of Biological Chemistry 2003 Mar; 278(13):11182.
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STAP-2/BKS, an adaptor/docking protein, modulates STAT3 activation in acute-phase response through its YXXQ motif.
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