HAND2 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of HAND2 polyclonal antibody (Cat # PAB4702) in NCI-H460 cell lysate (35 ug/lane). HAND2 (arrow) was detected using the purified polyclonal antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with HAND2 polyclonal antibody (Cat # PAB4702) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of HAND2.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human HAND2.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
ELISA (1:1000)
Western Blot (1:50-200)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of HAND2 polyclonal antibody (Cat # PAB4702) in NCI-H460 cell lysate (35 ug/lane). HAND2 (arrow) was detected using the purified polyclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with HAND2 polyclonal antibody (Cat # PAB4702) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.Enzyme-linked Immunoabsorbent Assay
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Gene Info — HAND2
Entrez GeneID
9464Protein Accession#
HAN2_HUMANGene Name
HAND2
Gene Alias
DHAND2, FLJ16260, Hed, MGC125303, MGC125304, Thing2, bHLHa26, dHand
Gene Description
heart and neural crest derivatives expressed 2
Omim ID
602407Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the basic helix-loop-helix family of transcription factors. This gene product is one of two closely related family members, the HAND proteins, which are asymmetrically expressed in the developing ventricular chambers and play an essential role in cardiac morphogenesis. Working in a complementary fashion, they function in the formation of the right ventricle and aortic arch arteries, implicating them as mediators of congenital heart disease. In addition, this transcription factor plays an important role in limb and branchial arch development. [provided by RefSeq
Other Designations
basic helix-loop-helix transcription factor HAND2
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Interactome
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Disease
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Publication Reference
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A GATA-dependent right ventricular enhancer controls dHAND transcription in the developing heart.
McFadden DG, Charite J, Richardson JA, Srivastava D, Firulli AB, Olson EN.
Development (Cambridge, England) 2000 Dec; 127(24):5331.
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Molecular cloning of the human HAND2 gene.
Russell MW, Kemp P, Wang L, Brody LC, Izumo S.
Biochimica et Biophysica Acta 1998 Dec; 1443(3):393.
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Heart and extra-embryonic mesodermal defects in mouse embryos lacking the bHLH transcription factor Hand1.
Firulli AB, McFadden DG, Lin Q, Srivastava D, Olson EN.
Nature Genetics 1998 Mar; 18(3):266.
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A GATA-dependent right ventricular enhancer controls dHAND transcription in the developing heart.
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