MAP4K2 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
The MAP4K2 polyclonal antibody (Cat # PAB4603) is used in Western blot to detect MAP4K2 in mouse skeletal muscle tissue lysate .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with MAP4K2 polyclonal antibody (Cat # PAB4603) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . HC = hepatocarcinoma .
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of MAP4K2.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to internal region of human MAP4K2.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Recommend Usage
ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
The MAP4K2 polyclonal antibody (Cat # PAB4603) is used in Western blot to detect MAP4K2 in mouse skeletal muscle tissue lysate .Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with MAP4K2 polyclonal antibody (Cat # PAB4603) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . HC = hepatocarcinoma .Enzyme-linked Immunoabsorbent Assay
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Gene Info — MAP4K2
Entrez GeneID
5871Protein Accession#
M4K2_HUMANGene Name
MAP4K2
Gene Alias
BL44, GCK, RAB8IP
Gene Description
mitogen-activated protein kinase kinase kinase kinase 2
Omim ID
603166Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the serine/threonine protein kinase family. Although this kinase is found in many tissues, its expression in lymphoid follicles is restricted to the cells of germinal centre, where it may participate in B-cell differentiation. This kinase can be activated by TNF-alpha, and has been shown to specifically activate MAP kinases. This kinase is also found to interact with TNF receptor-associated factor 2 (TRAF2), which is involved in the activation of MAP3K1/MEKK1. [provided by RefSeq
Other Designations
B lymphocyte serine/threonine protein kinase|Rab8 interacting protein|germinal centre kinase (GC kinase)
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Interactome
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Pathway
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Disease
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Publication Reference
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Direct activation of mitogen-activated protein kinase kinase kinase MEKK1 by the Ste20p homologue GCK and the adapter protein TRAF2.
Chadee DN, Yuasa T, Kyriakis JM.
Molecular and Cellular Biology 2002 Feb; 22(3):737.
Application：IP, WB, Human, RAMOS cells.
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Differential expression of a novel protein kinase in human B lymphocytes. Preferential localization in the germinal center.
Katz P, Whalen G, Kehrl JH.
The Journal of Biological Chemistry 1994 Jun; 269(24):16802.
Application：WB-Ce, WB-Ti, Human, Human B Lymphocytes.
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Direct activation of mitogen-activated protein kinase kinase kinase MEKK1 by the Ste20p homologue GCK and the adapter protein TRAF2.
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