HDGF polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of HDGF polyclonal antibody (Cat # PAB4472) in HeLa cell line lysates (35 ug/lane). HDGF (arrow) was detected using the purified HDGF polyclonal antibody (Cat # PAB4472) (1 : 60 dilution).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma reacted with HDGF polyclonal antibody (Cat # PAB4472) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated .
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of HDGF.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human HDGF.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Ammonium sulfate precipitation
Recommend Usage
ELISA (1:1000)
Western Blot (1:50-100)
Immunohistochemistry (1:10-50)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of HDGF polyclonal antibody (Cat # PAB4472) in HeLa cell line lysates (35 ug/lane). HDGF (arrow) was detected using the purified HDGF polyclonal antibody (Cat # PAB4472) (1 : 60 dilution).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma reacted with HDGF polyclonal antibody (Cat # PAB4472) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated .Enzyme-linked Immunoabsorbent Assay
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Gene Info — HDGF
Entrez GeneID
3068Protein Accession#
NP_004485;P51858Gene Name
HDGF
Gene Alias
DKFZp686J1764, FLJ96580, HMG1L2
Gene Description
hepatoma-derived growth factor (high-mobility group protein 1-like)
Omim ID
600339Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the hepatoma-derived growth factor family. The encoded protein has mitogenic and DNA-binding activity and may play a role in cellular proliferation and differentiation. This gene was thought initially to be located on chromosome X, however, that location has been determined to correspond to a related pseudogene. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq
Other Designations
OTTHUMP00000038721|hepatoma-derived growth factor
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Interactome
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Publication Reference
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SUMOylation of the hepatoma-derived growth factor negatively influences its binding to chromatin.
Thakar K, Niedenthal R, Okaz E, Franken S, Jakobs A, Gupta S, Kelm S, Dietz F.
FEBS Letters 2008 Apr; 275(7):1411.
Application：IF, WB-Tr, Monkey, COS-7 cells.
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Hepatoma-derived growth factor is a novel prognostic factor for gastrointestinal stromal tumors.
Chang KC, Tai MH, Lin JW, Wang CC, Huang CC, Hung CH, Chen CH, Lu SN, Lee CM, Changchien CS, Hu TH.
International Journal of Cancer 2007 Sep; 121(5):1059.
Application：IHC-P, Human, Human gastrointestinal stromal tumors.
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Expression level of hepatoma-derived growth factor correlates with tumor recurrence of esophageal carcinoma.
Yamamoto S, Tomita Y, Hoshida Y, Morii E, Yasuda T, Doki Y, Aozasa K, Uyama H, Nakamura H, Monden M.
Annals of Surgical Oncology 2007 Jul; 14(7):2141.
Application：IHC-P, Human, Human esophageal carcinoma.
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SUMOylation of the hepatoma-derived growth factor negatively influences its binding to chromatin.
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