MARCKS polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Western blot analysis of HeLa cell lysate (35 ug/lane) with MARCKS polyclonal antibody (Cat # PAB4366).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of MARCKS.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human MARCKS.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
ELISA (1:1000)
Western Blot (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of HeLa cell lysate (35 ug/lane) with MARCKS polyclonal antibody (Cat # PAB4366).Enzyme-linked Immunoabsorbent Assay
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Gene Info — MARCKS
Entrez GeneID
4082Protein Accession#
MACS_HUMANGene Name
MARCKS
Gene Alias
80K-L, FLJ14368, FLJ90045, MACS, PKCSL, PRKCSL
Gene Description
myristoylated alanine-rich protein kinase C substrate
Omim ID
177061Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a substrate for protein kinase C. It is localized to the plasma membrane and is an actin filament crosslinking protein. Phosphorylation by protein kinase C or binding to calcium-calmodulin inhibits its association with actin and with the plasma membrane, leading to its presence in the cytoplasm. The protein is thought to be involved in cell motility, phagocytosis, membrane trafficking and mitogenesis. [provided by RefSeq
Other Designations
OTTHUMP00000017045|myristoylated alanine-rich protein kinase C substrate (MARCKS, 80K-L)|phosphomyristin
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Interactome
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Pathway
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Publication Reference
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The MARCKS family of protein kinase-C substrates.
Aderem A.
Biochemical Society Transactions 1995 Aug; 23(3):587.
Application：IF, WB-Ce, WB-Tr, Human, Rat, Macrophages, PC-12 cells.
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Subregional mapping of 8 single copy loci to chromosome 6 by fluorescence in situ hybridization.
Rao PH, Murty VV, Gaidano G, Hauptschein R, Dalla-Favera R, Chaganti RS.
Cytogenetics and Cell Genetics 1994 Jan; 66(4):272.
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The MARCKS family of protein kinase-C substrates.
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