DOK1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of DOK1 polyclonal antibody (Cat # PAB3405) in HL-60 cell lysate. DOK1 (Arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with DOK1 polyclonal antibody (Cat # PAB3405) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of DOK1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human DOK1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-100)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of DOK1 polyclonal antibody (Cat # PAB3405) in HL-60 cell lysate. DOK1 (Arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with DOK1 polyclonal antibody (Cat # PAB3405) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma. -
Gene Info — DOK1
Entrez GeneID
1796Protein Accession#
Q99704Gene Name
DOK1
Gene Alias
MGC117395, MGC138860, P62DOK
Gene Description
docking protein 1, 62kDa (downstream of tyrosine kinase 1)
Omim ID
602919Gene Ontology
HyperlinkGene Summary
Docking protein 1 is constitutively tyrosine phosphorylated in hematopoietic progenitors isolated from chronic myelogenous leukemia (CML) patients in the chronic phase. It may be a critical substrate for p210(bcr/abl), a chimeric protein whose presence is associated with CML. Docking protein 1 contains a putative pleckstrin homology domain at the amino terminus and ten PXXP SH3 recognition motifs. Docking protein 2 binds p120 (RasGAP) from CML cells. It has been postulated to play a role in mitogenic signaling. [provided by RefSeq
Other Designations
Downstream of tyrosine kinase 1|docking protein 1|docking protein 1 (downstream of tyrosine kinase 1)|docking protein 1, 62kD (downstream of tyrosine kinase 1)
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Interactome
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Disease
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Publication Reference
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The rasGAP-binding protein, Dok-1, mediates activin signaling via serine/threonine kinase receptors.
Yamakawa N, Tsuchida K, Sugino H.
The EMBO Journal 2002 Apr; 21(7):1684.
Application：IP, WB-Tr, Human, Mouse, Monkey, COS-7, HEK 293, HS72 cells.
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Phosphatidylinositol 3-kinase and Src family kinases are required for phosphorylation and membrane recruitment of Dok-1 in c-Kit signaling.
Liang X, Wisniewski D, Strife A, Shivakrupa, Clarkson B, Resh MD.
The Journal of Biological Chemistry 2002 Apr; 277(16):13732.
Application：IP, WB-Tr, Monkey, Mouse, COS-1, Mo7, Bone marrow mast cells.
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Molecular cloning of a truncated p62Dok1 isoform, p22Dok(del).
Hubert P, Ferreira V, Debre P, Bismuth G.
European Journal of Immunogenetics 2000 Jun; 27(3):145.
Application：WB-Tr, Human, Jurkat cells.
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The rasGAP-binding protein, Dok-1, mediates activin signaling via serine/threonine kinase receptors.
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