DOK1 (phospho S450) polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot of Jurkat cells stimulated with calyculin A (100 nM, 30 min) (lane 1) followed by lambda phosphatase (lane 2) treatment. The blots were probed with DOK1 (phospho S450) polyclonal antibody (Cat # PAB7908).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of DOK1.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S450 of human DOK1.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This sequence is conserved in Dok1 from rat (Ser-449) and mouse (Ser-451). The site is not conserved in other Dok family members.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:2000)
Western Blot (1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1 mg/mL BSA, 0.05% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot of Jurkat cells stimulated with calyculin A (100 nM, 30 min) (lane 1) followed by lambda phosphatase (lane 2) treatment. The blots were probed with DOK1 (phospho S450) polyclonal antibody (Cat # PAB7908).Enzyme-linked Immunoabsorbent Assay
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Gene Info — DOK1
Entrez GeneID
1796Gene Name
DOK1
Gene Alias
MGC117395, MGC138860, P62DOK
Gene Description
docking protein 1, 62kDa (downstream of tyrosine kinase 1)
Omim ID
602919Gene Ontology
HyperlinkGene Summary
Docking protein 1 is constitutively tyrosine phosphorylated in hematopoietic progenitors isolated from chronic myelogenous leukemia (CML) patients in the chronic phase. It may be a critical substrate for p210(bcr/abl), a chimeric protein whose presence is associated with CML. Docking protein 1 contains a putative pleckstrin homology domain at the amino terminus and ten PXXP SH3 recognition motifs. Docking protein 2 binds p120 (RasGAP) from CML cells. It has been postulated to play a role in mitogenic signaling. [provided by RefSeq
Other Designations
Downstream of tyrosine kinase 1|docking protein 1|docking protein 1 (downstream of tyrosine kinase 1)|docking protein 1, 62kD (downstream of tyrosine kinase 1)
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Interactome
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Disease
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Publication Reference
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IkappaB kinase beta phosphorylates Dok1 serines in response to TNF, IL-1, or gamma radiation.
Lee S, Andrieu C, Saltel F, Destaing O, Auclair J, Pouchkine V, Michelon J, Salaun B, Kobayashi R, Jurdic P, Kieff ED, Sylla BS.
PNAS 2004 Dec; 101(50):17416.
Application：WB, Human, HeLa, HEK 293T cells.
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c-Abl phosphorylates Dok1 to promote filopodia during cell spreading.
Woodring PJ, Meisenhelder J, Johnson SA, Zhou GL, Field J, Shah K, Bladt F, Pawson T, Niki M, Pandolfi PP, Wang JY, Hunter T.
The Journal of Cell Biology 2004 May; 165(4):493.
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Tyrosine phosphorylation of p62dok by p210bcr-abl inhibits RasGAP activity.
Kashige N, Carpino N, Kobayashi R.
PNAS 2000 Feb; 97(5):2093.
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IkappaB kinase beta phosphorylates Dok1 serines in response to TNF, IL-1, or gamma radiation.
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