WWTR1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of mouse cell line NIH-3T3 and rat cell line NBT-II.
Western Blot (Transfected lysate)
Western blot analysis of EFO-21 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using WWTR1 polyclonal antibody (Cat # PAB31829). Loading control: Anti-PPIB
Immunohistochemistry
Immunohistochemical staining of human placenta shows strong nuclear positivity in endothelial cells.
Immunohistochemistry
Immunohistochemical staining of human skeletal muscle shows weak positivity in myocytes.
Immunohistochemistry
Immunohistochemical staining of human colon shows strong nuclear positivity in a subset of lymphoid cells.
Immunohistochemistry
Immunohistochemical staining of human kidney shows moderate nuclear positivity in glomerular cells and a subset of cells in distal tubules.
Immunohistochemistry
Immunohistochemical staining of human renal cancer shows strong nuclear positivity in tumor cells.
Immunofluorescence
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm, nuclear bodies and cytosol.
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant human WWTR1.
Sequence
MNPKPSSWRKKILPESFFKEPDSGSHSRQSSTDSSGGHPGPRLAGGAQHVRSHSSPASLQLGTGAGAAGSPAQQHAHLRQQSYDVTDELPLPPGWEMTFTATGQRYFLNHIEKITTWQDPRKAMNQPLNHMNLHPAVSST
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody reacts to WWTR1.
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence (0.25-2 ug/mL)
Immunohistochemistry (1:200 - 1:500)
Western Blot (0.04-0.4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of mouse cell line NIH-3T3 and rat cell line NBT-II.Western Blot (Transfected lysate)
Western blot analysis of EFO-21 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using WWTR1 polyclonal antibody (Cat # PAB31829). Loading control: Anti-PPIBImmunohistochemistry
Immunohistochemical staining of human placenta shows strong nuclear positivity in endothelial cells.Immunohistochemistry
Immunohistochemical staining of human skeletal muscle shows weak positivity in myocytes.Immunohistochemistry
Immunohistochemical staining of human colon shows strong nuclear positivity in a subset of lymphoid cells.Immunohistochemistry
Immunohistochemical staining of human kidney shows moderate nuclear positivity in glomerular cells and a subset of cells in distal tubules.Immunohistochemistry
Immunohistochemical staining of human renal cancer shows strong nuclear positivity in tumor cells.Immunofluorescence
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm, nuclear bodies and cytosol. -
Gene Info — WWTR1
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Interactome
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Disease
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