SERPINB5 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Western Blot analysis of human esophagus tissue.
Western Blot (Cell lysate)
Western Blot analysis of (1) NIH-3T3 cell lysate (Mouse embryonic fibroblast cells), and (2) NBT-II cell lysate (Rat Wistar bladder tumour cells).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human esophagus shows strong cytoplasmic positivity in squamous epithelial cells.
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant human SERPINB5.
Immunogen
Recombinant protein corresponding to amino acids 203-285 of human SERPINB5.
Sequence
TFCMGNIDSINCKIIELPFQNKHLSMFILLPKDVEDESTGLEKIEKQLNSESLSQWTNPSTMANAKVKLSIPKFKVEKMIDPK
Host
Rabbit
Reactivity
Human, Rat
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200-1:500)
Western Blot (1:100-1:250)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C for short term storage. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western Blot analysis of human esophagus tissue.Western Blot (Cell lysate)
Western Blot analysis of (1) NIH-3T3 cell lysate (Mouse embryonic fibroblast cells), and (2) NBT-II cell lysate (Rat Wistar bladder tumour cells).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human esophagus shows strong cytoplasmic positivity in squamous epithelial cells. -
Gene Info — SERPINB5
Entrez GeneID
5268Protein Accession#
P36952Gene Name
SERPINB5
Gene Alias
PI5, maspin
Gene Description
serpin peptidase inhibitor, clade B (ovalbumin), member 5
Omim ID
154790Gene Ontology
HyperlinkGene Summary
clade B (ovalbumin)
Other Designations
protease inhibitor 5 (maspin)|serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 5
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Interactome
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Pathway
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Publication Reference
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EGFR Signaling Regulates Maspin/SerpinB5 Phosphorylation and Nuclear Localization in Mammary Epithelial Cells.
Tamazato Longhi M, Magalhaes M, Reina J, Morais Freitas V, Cella N.
PLoS One 2016 Jul; 11(7):e0159856.
Application：IF, IHC-P, WB-Ce, WB-Ti, WB-Re, Human Mouse, MCF-10A cells, Mouse inguinal glands, Mouse mammary glands, Recombinant proteins.
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EGFR Signaling Regulates Maspin/SerpinB5 Phosphorylation and Nuclear Localization in Mammary Epithelial Cells.
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