DDX3X polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of DDX3X.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human DDX3X.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:10-50)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of DDX3X polyclonal antibody (Cat # PAB3151) in HeLa cell line lysates (35 ug/lane). DDX3X (arrow) was detected using the purified polyclonal antibody.Western Blot (Transfected lysate)
Western blot analysis of DDX3X (arrow) using rabbit DDX3X polyclonal antibody (Cat # PAB3151). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the DDX3 gene (Lane 2) (Origene Technologies).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma reacted with DDX3X polyclonal antibody (Cat # PAB3151) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. -
Gene Info — DDX3X
Entrez GeneID
1654Protein Accession#
NP_001347;O00571Gene Name
DDX3X
Gene Alias
DBX, DDX14, DDX3, HLP2
Gene Description
DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked
Omim ID
300160Gene Ontology
HyperlinkGene Summary
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which interacts specifically with hepatitis C virus core protein resulting a change in intracellular location. This gene has a homolog located in the nonrecombining region of the Y chromosome. The protein sequence is 91% identical between this gene and the Y-linked homolog. [provided by RefSeq
Other Designations
ATP-dependent RNA helicase DDX3X|CAP-Rf|DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 3|DEAD/H box-3|helicase like protein 2
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Interactome
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Disease
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Publication Reference
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DDX3 DEAD-box RNA helicase is required for hepatitis C virus RNA replication.
Ariumi Y, Kuroki M, Abe K, Dansako H, Ikeda M, Wakita T, Kato N.
Journal of Virology 2007 Dec; 81(24):13922.
Application:IF, WB-Tr, Human, 293FT cells.
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Crystal structure of conserved domains 1 and 2 of the human DEAD-box helicase DDX3X in complex with the mononucleotide AMP.
Hogbom M, Collins R, van den Berg S, Jenvert RM, Karlberg T, Kotenyova T, Flores A, Karlsson Hedestam GB, Schiavone LH.
Journal of Molecular Biology 2007 Sep; 372(1):150.
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Human DEAD-box ATPase DDX3 shows a relaxed nucleoside substrate specificity.
Franca R, Belfiore A, Spadari S, Maga G.
Proteins 2007 Jun; 67(4):1128.
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DDX3 DEAD-box RNA helicase is required for hepatitis C virus RNA replication.
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