DDX3X monoclonal antibody, clone 3B9G8
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of (1) HEK293 cells, (2) DDX3X-hIgGFc transfected HEK293 cell lysate.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of DDX3X monoclonal antibody, clone 3B9G8.
Flow Cytometry
Flow cytometric analysis of HeLa cells with DDX3X monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human DDX3X.
Immunogen
Recombinant protein corresponding to amino acid 518-661 of human DDX3X from E. coli.
Host
Mouse
Theoretical MW (kDa)
73.2kDa
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry
Immunocytochemistry
Flow Cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of (1) HEK293 cells, (2) DDX3X-hIgGFc transfected HEK293 cell lysate.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of DDX3X monoclonal antibody, clone 3B9G8.Flow Cytometry
Flow cytometric analysis of HeLa cells with DDX3X monoclonal antibody (green) and negative control (red). -
Gene Info — DDX3X
Entrez GeneID
1654Gene Name
DDX3X
Gene Alias
DBX, DDX14, DDX3, HLP2
Gene Description
DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked
Omim ID
300160Gene Ontology
HyperlinkGene Summary
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which interacts specifically with hepatitis C virus core protein resulting a change in intracellular location. This gene has a homolog located in the nonrecombining region of the Y chromosome. The protein sequence is 91% identical between this gene and the Y-linked homolog. [provided by RefSeq
Other Designations
ATP-dependent RNA helicase DDX3X|CAP-Rf|DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 3|DEAD/H box-3|helicase like protein 2
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Interactome
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Disease
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