ATG10 polyclonal antibody
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More Files
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human stomach with ATG10 polyclonal antibody (Cat # PAB30243) shows moderate membranous and cytoplasmic positivity in glandular cells at 1:50 - 1:200 dilution.
Immunofluorescence
Immunofluorescent staining of human cell line MCF-7 with ATG10 polyclonal antibody (Cat # PAB30243) shows positivity in nucleus and nucleoli under 1-4 ug/mL concentration. Antibody staining is shown in green.
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant human ATG10.
Immunogen
Recombinant protein corresponding to amino acids of human ATG10.
Sequence
IGDSWEWRPSKDCSDGYMCKIHFQIKNGSVMSHLGASTHGQTCLPMEEAFELPLDDCEVIETAAASEVIKYEYHVLYSCSYQVPVLYFRASFLDGRPLTLKDIWEGVHECYKM
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-1:200)
Immunofluorescence (1-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human stomach with ATG10 polyclonal antibody (Cat # PAB30243) shows moderate membranous and cytoplasmic positivity in glandular cells at 1:50 - 1:200 dilution.Immunofluorescence
Immunofluorescent staining of human cell line MCF-7 with ATG10 polyclonal antibody (Cat # PAB30243) shows positivity in nucleus and nucleoli under 1-4 ug/mL concentration. Antibody staining is shown in green. -
Gene Info — ATG10
Entrez GeneID
83734Protein Accession#
Q9H0Y0Gene Name
ATG10
Gene Alias
APG10, APG10L, DKFZp586I0418, FLJ13954, pp12616
Gene Description
ATG10 autophagy related 10 homolog (S. cerevisiae)
Omim ID
610800Gene Ontology
HyperlinkGene Summary
Autophagy is a process for the bulk degradation of cytosolic compartments by lysosomes. ATG10 is an E2-like enzyme involved in 2 ubiquitin-like modifications essential for autophagosome formation: ATG12 (MIM 609608)-ATG5 (MIM 604261) conjugation and modification of a soluble form of MAP-LC3 (MAP1LC3A; MIM 601242), a homolog of yeast Apg8, to a membrane-bound form (Nemoto et al., 2003 [PubMed 12890687]).[supplied by OMIM
Other Designations
APG10 autophagy 10-like
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Interactome
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Disease
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