Hspd1 polyclonal antibody
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More Files
- More Functions
Western Blot
Western blot analysis of Lane 1: rat brain tissue lysate, Lane 2: rat liver tissue lysate, Lane 3: CEM cell lysate, Lane 4: HELA cell lysate, Lane 5: SMMC cell lysate, Lane 6: COLO320 cell lysate with Hspd1 polyclonal antibody (Cat# PAB29783) under 0.1-0.5 ug/mL working concentration.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of rat intestine tissue with Hspd1 polyclonal antibody (Cat# PAB29783) under 0.5-1 ug/mL working concentration.
Immunocytochemistry
Immunocytochemistry staining of SMMC cell with Hspd1 polyclonal antibody (Cat# PAB29783) under 0.5-1 ug/mL working concentration.
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Specification
Product Description
Rabbit polyclonal antibody raised against partial synthetic peptide of rat Hspd1.
Immunogen
A synthetic peptide corresponding to amino acids 503-516 at C-terminus of rat Hspd1.
Sequence
YDAMLGDFVNMVEK
Host
Rabbit
Theoretical MW (kDa)
60
Reactivity
Human, Mouse, Rat
Specificity
The synthetic peptide of this antibody is different from the related human sequence by one amino acid.
Form
Lyophilized
Purification
Immunogen affinity purification
Isotype
IgG
Recommend Usage
Immunocytochemistry (0.5-1 ug/mL)
Immunohistochemistry (0.5-1 ug/mL)
Western Blot (0.1-0.5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg sodium azide.
Storage Instruction
Store at -20°C.
After reconstitution with 0.2 mL of distilled water and concentration will be 500 ug/mL, store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide and thimerosol: POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: rat brain tissue lysate, Lane 2: rat liver tissue lysate, Lane 3: CEM cell lysate, Lane 4: HELA cell lysate, Lane 5: SMMC cell lysate, Lane 6: COLO320 cell lysate with Hspd1 polyclonal antibody (Cat# PAB29783) under 0.1-0.5 ug/mL working concentration.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of rat intestine tissue with Hspd1 polyclonal antibody (Cat# PAB29783) under 0.5-1 ug/mL working concentration.Immunocytochemistry
Immunocytochemistry staining of SMMC cell with Hspd1 polyclonal antibody (Cat# PAB29783) under 0.5-1 ug/mL working concentration. -
Gene Info — Hspd1
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